Translational_Unit

Part:BBa_I716339:Design

Designed by: nhu nguyen   Group: iGEM07_Berkeley_UC   (2007-08-14)


rbs-thpB-rbs-ThpC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 329
    Illegal NotI site found at 772
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1712
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal NgoMIV site found at 183
    Illegal NgoMIV site found at 807
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 63
    Illegal BsaI.rc site found at 654
    Illegal BsaI.rc site found at 1176
    Illegal BsaI.rc site found at 1433
    Illegal BsaI.rc site found at 1457
    Illegal BsaI.rc site found at 1532
    Illegal SapI.rc site found at 1236


Design Notes

Digest I716337 (BglII/XhoI, 2057bp, 428bp, S) Sub into I716336 (BamHI/XhoI, 3340bp, 9bp, L) Product is I716339


Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References